Hotspot

rpoC1-trnD-GUC

LSC · 22972-27389 · 4418 bp

Back to Hymenasplenium

Pi 0.0288

MarkerSeek score 63.30

Primer available yes

Label rpoC1-trnD-GUC

Feature Metadata

label_name: rpoC1-trnD-GUC
feature_type: igs
region: LSC
length_bp: 4418
variable_sites: 372
pi: 0.0288
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-trnD-GUC_p1	GAAGCAGAAAGGGGGAAACA	TGATTTGAGTCTATGTAGTAATTGCT	50.0	30.8	58.0	56.5	304-311 bp	0.5	31.1
rpoC1-trnD-GUC_p2	ACGGGCATATTTCCACTTCC	TGATTTGAGTCTATGTAGTAATTGCT	50.0	30.8	57.9	56.5	837-847 bp	0.5	28.9
rpoC1-trnD-GUC_p3	CAAGTTCTGGTAGTACGGGC	TGATTTGAGTCTATGTAGTAATTGCT	55.0	30.8	58.0	56.5	851-861 bp	0.5	28.8
rpoC1-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	TGATTTGAGTCTATGTAGTAATTGCT	55.0	30.8	58.0	56.5	852-862 bp	0.5	28.8
rpoC1-trnD-GUC_p5	TCTGTGCTATCCGTCGTAGT	TGATTTGAGTCTATGTAGTAATTGCT	50.0	30.8	58.0	56.5	502-507 bp	0.333333	22.5