Hotspot

psbK-psbI

LSC · 7049-7638 · 590 bp

Back to Hymenasplenium

Pi 0.0619

MarkerSeek score 81.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 101
pi: 0.0619
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GGGTCAACCAATTGAAGGGT	GAGAGATGGCTGAGAGGTCT	50.0	55.0	58.0	57.9	1129-1152 bp	1.0	88.5
psbK-psbI_p2	GGGTCAACCAATTGAAGGGT	CAGTTCTTCGGAGAGATGGC	50.0	55.0	58.0	58.1	1139-1152 bp	0.5	65.1
psbK-psbI_p3	GGGTCAACCAATTGAAGGGT	GTTCTTCGGAGAGATGGCTG	50.0	55.0	58.0	58.1	1137-1150 bp	0.5	65.1
psbK-psbI_p4	CACTTCGGGTTTCCTGTCAA	GTAACGTGATACCTGGGCAG	50.0	55.0	58.0	58.1	1487-1499 bp	0.5	64.9
psbK-psbI_p5	GGGTCAACCAATTGAAGGGT	GTAACGTGATACCTGGGCAG	50.0	55.0	58.0	58.1	1249-1262 bp	0.5	64.8