Hotspot

matK-rps16

LSC · 2232-3426 · 1195 bp

Back to Hymenasplenium

Pi 0.0583
MarkerSeek score 81.90
Primer available yes
Label matK-rps16

Feature Metadata

label_name
matK-rps16
feature_type
igs
region
LSC
length_bp
1195
variable_sites
200
pi
0.0583
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rps16_p1 TCCTCTCCGAGACCAAAGTT TCAATGAGAACGAGCTCTGC 50.0 50.0 58.0 58.0 1990 bp 0.083333 43.2
matK-rps16_p2 TTCCTCTCCGAGACCAAAGT TCAATGAGAACGAGCTCTGC 50.0 50.0 58.0 58.0 1991 bp 0.083333 43.2
matK-rps16_p3 TCCTCTCCGAGACCAAAGTT AATGAGAACGAGCTCTGCTG 50.0 50.0 58.0 58.0 1988 bp 0.083333 43.2
matK-rps16_p4 TCCTCTCCGAGACCAAAGTT CAATGAGAACGAGCTCTGCT 50.0 50.0 58.0 58.0 1989 bp 0.083333 43.2
matK-rps16_p5 TTCCTCTCCGAGACCAAAGT AATGAGAACGAGCTCTGCTG 50.0 50.0 58.0 58.0 1989 bp 0.083333 43.2