Hotspot

ndhD-psaC

SSC · 122254-122563 · 310 bp

Back to Hymenaea

Pi 0.0613

MarkerSeek score 82.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 310
variable_sites: 19
pi: 0.0613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTGGTCCGATAGAGAGTCCA	CCGACGGATTTCTTGAGTGT	50.0	50.0	57.2	57.9	467 bp	1.0	76.0
ndhD-psaC_p2	TGGTCCGATAGAGAGTCCAT	CCGACGGATTTCTTGAGTGT	50.0	50.0	56.9	57.9	466 bp	1.0	74.2
ndhD-psaC_p3	TCCCGTGAGTAAAATTGGTCC	CCGACGGATTTCTTGAGTGT	47.6	50.0	57.9	57.9	481 bp	1.0	74.0
ndhD-psaC_p4	TTGGTCCGATAGAGAGTCCA	TCCGACGGATTTCTTGAGTG	50.0	50.0	57.2	57.6	468 bp	1.0	73.8
ndhD-psaC_p5	TTGGTCCGATAGAGAGTCCA	TGTCCGACGGATTTCTTGAG	50.0	50.0	57.2	57.6	470 bp	1.0	73.8