Hotspot

rpl32-trnL-UAG

SSC · 118330-119597 · 1268 bp

Back to Hymenaea

Pi 0.0201

MarkerSeek score 72.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1268
variable_sites: 25
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCCACATCTCTTTCTACGGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1363-1427 bp	1.0	82.4
rpl32-trnL-UAG_p2	GCCACATCTCTTTCTACGGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	1364-1428 bp	1.0	82.4
rpl32-trnL-UAG_p3	ACGGGGAACTCAAAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1348-1412 bp	1.0	82.4
rpl32-trnL-UAG_p4	ACGGGGAACTCAAAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1349-1413 bp	1.0	82.3
rpl32-trnL-UAG_p5	GCCACATCTCTTTCTACGGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	1373-1437 bp	1.0	81.4