Hotspot

rps4-trnT-UGU

LSC · 49357-49882 · 526 bp

Back to Hymenaea

Pi 0.0429

MarkerSeek score 77.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 21
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAAGACCCCAGACGACGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	623-643 bp	1.0	84.0
rps4-trnT-UGU_p2	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	596-616 bp	1.0	83.5
rps4-trnT-UGU_p3	TAAAGACCCCAGACGACGTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.8	57.8	591-611 bp	1.0	83.3
rps4-trnT-UGU_p4	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	601-621 bp	1.0	82.8
rps4-trnT-UGU_p5	GACCCCAGACGACGTATTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.6	57.9	619-639 bp	1.0	82.6