Hotspot

trnG-GCC-trnfM-CAU

LSC · 39858-40146 · 289 bp

Back to Hymenaea

Pi 0.0638

MarkerSeek score 75.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 15
pi: 0.0638
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	314-367 bp	1.0	85.9
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	312-365 bp	1.0	84.8
trnG-GCC-trnfM-CAU_p3	GGGTTCAATTCCCGCTATCC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.4	58.0	293-346 bp	1.0	84.3
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	335-388 bp	1.0	84.2
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	334-387 bp	1.0	84.2