Hotspot

trnC-GCA-petN

LSC · 29480-30270 · 791 bp

Back to Hymenaea

Pi 0.0273

MarkerSeek score 70.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 21
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	847-872 bp	1.0	82.0
trnC-GCA-petN_p2	TTTATCGTTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.7	58.2	900-925 bp	1.0	80.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	865-890 bp	1.0	79.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACCAGGG	50.0	55.0	58.1	57.5	898-923 bp	1.0	79.1
trnC-GCA-petN_p5	TTTATCGTTTTGGCGGCATG	TCTTCCCCATACTACCAGGG	45.0	55.0	57.7	57.5	951-976 bp	1.0	77.4