Hotspot

trnH-GUG-psbA

LSC · 93-523 · 431 bp

Back to Hymenaea

Pi 0.0274

MarkerSeek score 75.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 11
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCCAT	55.0	55.0	58.1	58.0	497-502 bp	1.0	84.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	511-516 bp	1.0	84.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	510-515 bp	1.0	84.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	512-517 bp	1.0	84.6
trnH-GUG-psbA_p5	TATGTTATGGGCGAACGACG	CCTCTAGACCTAGCTGCCAT	50.0	55.0	58.2	58.0	559-564 bp	1.0	84.2