Hotspot

ycf1

SSC · 121801-126963 · 5163 bp

Back to Hylotelephium

Pi 0.00883

MarkerSeek score 47.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5163
variable_sites: 107
pi: 0.00883
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTTGAGGGGTCGTTTATT	CCCCAAAACCAACCTTCCTT	35.0	50.0	53.0	58.2	175 bp	1.0	45.9
ycf1_p2	TTTTTGAGGGGTCGTTTATT	TCACCCCAAAACCAACCTTC	35.0	50.0	53.0	58.2	178 bp	1.0	45.8
ycf1_p3	TTTTTGAGGGGTCGTTTATT	TTCACCCCAAAACCAACCTT	35.0	45.0	53.0	57.7	179 bp	1.0	45.8
ycf1_p4	TTTTTGAGGGGTCGTTTATT	GTTCACCCCAAAACCAACCT	35.0	50.0	53.0	58.5	180 bp	1.0	45.8
ycf1_p5	TTTTTGAGGGGTCGTTTATT	TTTTAACTGTGTGGGGGAGG	35.0	50.0	53.0	57.3	219 bp	1.0	44.7