Hotspot

ndhA

SSC · 117648-119832 · 2185 bp

Back to Hylotelephium

Pi 0.00664

MarkerSeek score 44.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2185
variable_sites: 31
pi: 0.00664
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCAGCTCGTACTGTTTGTT	GTTTATCGGGCCCAATGCTA	45.0	50.0	58.1	58.0	2829-2850 bp	1.0	81.0
ndhA_p2	TGCAGCTCGTACTGTTTGTT	TTTATCGGGCCCAATGCTAC	45.0	50.0	58.1	58.0	2828-2849 bp	1.0	81.0
ndhA_p3	TTGCAGCTCGTACTGTTTGT	GTTTATCGGGCCCAATGCTA	45.0	50.0	58.1	58.0	2830-2851 bp	1.0	81.0
ndhA_p4	TTGCAGCTCGTACTGTTTGT	TTTATCGGGCCCAATGCTAC	45.0	50.0	58.1	58.0	2829-2850 bp	1.0	81.0
ndhA_p5	TGCAGCTCGTACTGTTTGTT	GCTACGAGCTTCCGGAATAG	45.0	55.0	58.1	57.9	2813-2834 bp	1.0	80.8