Hotspot

rpl32-trnL-UAG

SSC · 111920-112358 · 439 bp

Back to Hylotelephium

Pi 0.036

MarkerSeek score 65.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 439
variable_sites: 37
pi: 0.036
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCCTTTT	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	540-587 bp	1.0	88.1
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCCTTTT	TTAGAAGAGAATGTGCCGCC	45.0	50.0	57.9	58.0	567-614 bp	1.0	87.7
rpl32-trnL-UAG_p3	GGCAGCGTTAAAAGCCTTTT	AAGCACTGCTTCCTAAGAGC	45.0	50.0	57.9	57.9	523-570 bp	1.0	87.4
rpl32-trnL-UAG_p4	AAAAGGAAGGGGTATTGGGC	TTCGAACCGAGATGCTCAAG	50.0	50.0	57.8	58.0	557-604 bp	1.0	87.1
rpl32-trnL-UAG_p5	AAAAGGAAGGGGTATTGGGC	TTAGAAGAGAATGTGCCGCC	50.0	50.0	57.8	58.0	584-631 bp	1.0	86.7