Hotspot

ndhF-rpl32

SSC · 111176-111760 · 585 bp

Back to Hylotelephium

Pi 0.0123

MarkerSeek score 56.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 585
variable_sites: 21
pi: 0.0123
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCCCTAGTCCTATTCCCA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.1	57.9	760-779 bp	1.0	74.8
ndhF-rpl32_p2	CTGGAAGTGGAAGGAAAGGT	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.0	57.9	734-753 bp	1.0	74.4
ndhF-rpl32_p3	AAGCCCTAGTCCTATTCCCA	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.1	57.8	743-762 bp	1.0	74.1
ndhF-rpl32_p4	CTGGAAGTGGAAGGAAAGGT	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.0	57.8	717-736 bp	1.0	73.6
ndhF-rpl32_p5	AGCCCTAGTCCTATTCCCAT	AAAAGGCTTTTAACGCTGCC	50.0	45.0	56.9	57.9	759-778 bp	1.0	72.9