Hotspot

rpl20-rps12

LSC · 68024-68816 · 793 bp

Back to Hylotelephium

Pi 0.00131

MarkerSeek score 51.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTGTTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	862-867 bp	1.0	77.0
rpl20-rps12_p2	TTGTTTCTACGTCTCCGAGC	GCTCTTAGGGGATGTCCTCA	50.0	55.0	57.7	58.2	883-888 bp	1.0	76.3
rpl20-rps12_p3	TTGTTTCTACGTCTCCGAGC	TCTTAGGGGATGTCCTCAGC	50.0	55.0	57.7	58.2	881-886 bp	1.0	76.3
rpl20-rps12_p4	TGTTTCTACGTCTCCGAGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.5	57.9	861-866 bp	1.0	76.0
rpl20-rps12_p5	TGTTTCTACGTCTCCGAGCT	TCTTAGGGGATGTCCTCAGC	50.0	55.0	58.5	58.2	880-885 bp	1.0	75.3