Hotspot

psbE-petL

LSC · 63865-65038 · 1174 bp

Back to Hylotelephium

Pi 0.00869

MarkerSeek score 57.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 26
pi: 0.00869
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.9	58.0	1352-1369 bp	1.0	82.8
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1275-1292 bp	1.0	82.2
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	55.0	50.0	58.2	58.0	1270-1287 bp	1.0	81.8
psbE-petL_p4	ACCAACCCGCAATGAATAGG	CGTATCTTGCTCAGCCCAAT	50.0	50.0	58.2	58.0	1354-1371 bp	1.0	81.3
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AAGTCGTATCTTGCTCAGCC	50.0	50.0	57.9	57.7	1356-1373 bp	1.0	80.7