Hotspot

psbL-psbF

LSC · 63462-63483 · 22 bp

Back to Hylotelephium

Pi 0.0244

MarkerSeek score 50.40

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 1
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	CGTTTGGATTTGATTGTGTCGT	ATGGTTGGCTATTCACGGAC	40.9	50.0	58.1	58.0	126 bp	1.0	63.4
psbL-psbF_p2	CGTTTGGATTTGATTGTGTCGT	ACGGACTAGCTGTACCTACC	40.9	55.0	58.1	58.0	112 bp	1.0	63.4
psbL-psbF_p3	CGTTTGGATTTGATTGTGTCGT	GGACTAGCTGTACCTACCGT	40.9	55.0	58.1	58.0	110 bp	1.0	63.4
psbL-psbF_p4	TCGTTTGGATTTGATTGTGTCG	ATGGTTGGCTATTCACGGAC	40.9	50.0	57.9	58.0	127 bp	1.0	63.2
psbL-psbF_p5	TCGTTTGGATTTGATTGTGTCG	GGACTAGCTGTACCTACCGT	40.9	55.0	57.9	58.0	111 bp	1.0	63.2