Hotspot

accD-psaI

LSC · 58199-58753 · 555 bp

Back to Hylotelephium

Pi 0.0109

MarkerSeek score 40.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 555
variable_sites: 14
pi: 0.0109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATTGCATTTGCGGGTAAACG	TAAAAAGACCCGCGATACCG	45.0	50.0	58.0	58.1	1338-1349 bp	1.0	81.3
accD-psaI_p2	ATTGCATTTGCGGGTAAACG	CCAGGAAATCCCCAACGAAA	45.0	50.0	58.0	58.1	1444-1455 bp	1.0	81.1
accD-psaI_p3	ATTGCATTTGCGGGTAAACG	GATACAGGCCCAACCGAAAT	45.0	50.0	58.0	57.9	1198-1200 bp	0.5	60.9
accD-psaI_p4	GCTACCAAGAGCTTTCTCCC	GATACAGGCCCAACCGAAAT	55.0	50.0	58.0	57.9	1429-1431 bp	0.5	60.6
accD-psaI_p5	ACCAAGAGCTTTCTCCCTCT	GATACAGGCCCAACCGAAAT	50.0	50.0	58.0	57.9	1426-1428 bp	0.5	60.5