Hotspot

psbK-psbI

LSC · 7758-8161 · 404 bp

Back to Hylotelephium

Pi 0.00815

MarkerSeek score 55.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 10
pi: 0.00815
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	CTTCTCGTCCAGGATTACGTC	50.0	52.4	57.9	57.9	534-540 bp	1.0	73.4
psbK-psbI_p2	TGCCAGTTATACCCCTGCTA	CTTCTCGTCCAGGATTACGTC	50.0	52.4	57.8	57.9	572-578 bp	1.0	72.2
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTCTCGTCCAGGATTACGTC	50.0	50.0	57.9	56.8	533-539 bp	1.0	72.2
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	CTTCTCGTCCAGGATTACGT	50.0	50.0	57.9	56.8	534-540 bp	1.0	72.1
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TCTTCTCGTCCAGGATTACGT	50.0	47.6	57.9	58.3	535-541 bp	1.0	71.7