Hotspot

rps16-trnQ-UUG

LSC · 5888-7162 · 1275 bp

Back to Hylotelephium

Pi 0.0193

MarkerSeek score 60.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 55
pi: 0.0193
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	441-1363 bp	1.0	85.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	452-1374 bp	1.0	85.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTATGCGGAGGTTCGAATCC	45.0	55.0	58.1	58.2	448-1370 bp	1.0	84.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTATGCGGAGGTTCGAATCC	50.0	55.0	58.2	58.2	459-1381 bp	1.0	84.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	ATTTAAATGCAGTGGGGCGT	45.0	45.0	58.1	58.4	501-1424 bp	1.0	82.7