Hotspot

trnH-GUG-psbA

LSC · 73-311 · 239 bp

Back to Hylotelephium

Pi 0.0306

MarkerSeek score 68.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 17
pi: 0.0306
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	299-328 bp	1.0	85.7
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	302-331 bp	1.0	85.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	313-342 bp	1.0	85.4
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	312-341 bp	1.0	85.4
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	314-343 bp	1.0	85.3