primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	73	311	299-328	1.000000	85.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	73	311	302-331	1.000000	85.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	73	311	313-342	1.000000	85.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	73	311	312-341	1.000000	85.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	73	311	314-343	1.000000	85.3
psbA_p1	psbA	1	ACTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	24	20	37.5	50.0	56.3	58.4	0.0	0.0	6.039871	312	1373	1136-1136	1.000000	40.4
psbA_p2	psbA	2	AACTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	25	20	36.0	50.0	57.0	58.4	0.0	0.0	6.401124	312	1373	1137-1137	1.000000	40.4
psbA_p3	psbA	3	ACTCGTATAGACTAATACCGAAGTT	ATTTGGTTGCTTGGGAGTCC	25	20	36.0	50.0	57.0	58.4	0.0	0.0	6.401124	312	1373	1136-1136	1.000000	40.4
psbA_p4	psbA	4	ACTCGTATAGACTAATACCGAAG	ATTTGGTTGCTTGGGAGTCC	23	20	39.1	50.0	54.7	58.4	0.0	0.0	6.674927	312	1373	1136-1136	1.000000	40.4
psbA_p5	psbA	5	CTCGTATAGACTAATACCGAAGT	ATTTGGTTGCTTGGGAGTCC	23	20	39.1	50.0	54.7	58.4	0.0	0.0	6.674927	312	1373	1135-1135	1.000000	40.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5888	7162	441-1363	1.000000	85.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5888	7162	452-1374	1.000000	85.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTATGCGGAGGTTCGAATCC	20	20	45.0	55.0	58.1	58.2	8.1	4.4	0.354799	5888	7162	448-1370	1.000000	84.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTATGCGGAGGTTCGAATCC	20	20	50.0	55.0	58.2	58.2	0.0	4.4	0.419707	5888	7162	459-1381	1.000000	84.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	ATTTAAATGCAGTGGGGCGT	20	20	45.0	45.0	58.1	58.4	8.1	0.0	0.583357	5888	7162	501-1424	1.000000	82.7
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	CTTCTCGTCCAGGATTACGTC	20	21	50.0	52.4	57.9	57.9	1.4	0.0	1.123815	7758	8161	534-540	1.000000	73.4
psbK-psbI_p2	psbK-psbI	2	TGCCAGTTATACCCCTGCTA	CTTCTCGTCCAGGATTACGTC	20	21	50.0	52.4	57.8	57.9	0.0	0.0	1.251809	7758	8161	572-578	1.000000	72.2
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTCTCGTCCAGGATTACGTC	20	20	50.0	50.0	57.9	56.8	1.4	0.0	1.276163	7758	8161	533-539	1.000000	72.2
psbK-psbI_p4	psbK-psbI	4	GTTTGGCAAGCTGCTGTAAG	CTTCTCGTCCAGGATTACGT	20	20	50.0	50.0	57.9	56.8	1.4	0.0	1.284861	7758	8161	534-540	1.000000	72.1
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	TCTTCTCGTCCAGGATTACGT	20	21	50.0	47.6	57.9	58.3	1.4	0.0	1.336356	7758	8161	535-541	1.000000	71.7
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.2	55.6	0.0	0.0	4.584894	9904	10134	317-320	1.000000	48.6
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	TCCTGAGTTGACGTCGACTA	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	57.8	55.6	33.2	0.0	4.548780	9904	10134	344-347	1.000000	48.5
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.2	54.6	0.0	0.0	4.614976	9904	10134	311-314	1.000000	48.4
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	TCCTGAGTTGACGTCGACTA	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	57.8	54.6	33.2	0.0	4.578862	9904	10134	338-341	1.000000	48.3
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	TCCTGAGTTGACGTCGACTA	ACCAAAGGTTTAGAAGACCTATGT	20	24	50.0	37.5	57.8	57.6	33.2	0.0	4.624193	9904	10134	349-352	1.000000	47.8
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.0	58.0	0.0	0.0	0.031606	31571	32396	946-966	1.000000	83.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	20	20	60.0	55.0	58.0	58.1	0.0	0.0	0.089703	31571	32396	925-945	1.000000	82.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	20	20	60.0	50.0	58.0	58.1	0.0	0.0	0.111858	31571	32396	922-942	1.000000	82.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	20	20	60.0	55.0	58.0	58.2	0.0	0.0	0.176539	31571	32396	943-963	1.000000	82.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	31571	32396	911-931	1.000000	81.4
accD-psaI_p1	accD-psaI	1	ATTGCATTTGCGGGTAAACG	TAAAAAGACCCGCGATACCG	20	20	45.0	50.0	58.0	58.1	0.0	8.2	0.085340	58199	58753	1338-1349	1.000000	81.3
accD-psaI_p2	accD-psaI	2	ATTGCATTTGCGGGTAAACG	CCAGGAAATCCCCAACGAAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.092698	58199	58753	1444-1455	1.000000	81.1
accD-psaI_p3	accD-psaI	3	ATTGCATTTGCGGGTAAACG	GATACAGGCCCAACCGAAAT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.063352	58199	58753	1198-1200	0.500000	60.9
accD-psaI_p4	accD-psaI	4	GCTACCAAGAGCTTTCTCCC	GATACAGGCCCAACCGAAAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.084041	58199	58753	1429-1431	0.500000	60.6
accD-psaI_p5	accD-psaI	5	ACCAAGAGCTTTCTCCCTCT	GATACAGGCCCAACCGAAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090168	58199	58753	1426-1428	0.500000	60.5
psbL-psbF_p1	psbL-psbF	1	CGTTTGGATTTGATTGTGTCGT	ATGGTTGGCTATTCACGGAC	22	20	40.9	50.0	58.1	58.0	0.0	0.0	2.154217	63462	63483	126-126	1.000000	63.4
psbL-psbF_p2	psbL-psbF	2	CGTTTGGATTTGATTGTGTCGT	ACGGACTAGCTGTACCTACC	22	20	40.9	55.0	58.1	58.0	0.0	0.0	2.165719	63462	63483	112-112	1.000000	63.4
psbL-psbF_p3	psbL-psbF	3	CGTTTGGATTTGATTGTGTCGT	GGACTAGCTGTACCTACCGT	22	20	40.9	55.0	58.1	58.0	0.0	0.0	2.165719	63462	63483	110-110	1.000000	63.4
psbL-psbF_p4	psbL-psbF	4	TCGTTTGGATTTGATTGTGTCG	ATGGTTGGCTATTCACGGAC	22	20	40.9	50.0	57.9	58.0	0.0	0.0	2.178905	63462	63483	127-127	1.000000	63.2
psbL-psbF_p5	psbL-psbF	5	TCGTTTGGATTTGATTGTGTCG	GGACTAGCTGTACCTACCGT	22	20	40.9	55.0	57.9	58.0	0.0	0.0	2.190407	63462	63483	111-111	1.000000	63.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGCCCAAT	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.092293	63865	65038	1352-1369	1.000000	82.8
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.197301	63865	65038	1275-1292	1.000000	82.2
psbE-petL_p3	psbE-petL	3	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	20	20	55.0	50.0	58.2	58.0	0.9	0.0	0.238889	63865	65038	1270-1287	1.000000	81.8
psbE-petL_p4	psbE-petL	4	ACCAACCCGCAATGAATAGG	CGTATCTTGCTCAGCCCAAT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.278251	63865	65038	1354-1371	1.000000	81.3
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	AAGTCGTATCTTGCTCAGCC	20	20	50.0	50.0	57.9	57.7	6.4	0.0	0.352911	63865	65038	1356-1373	1.000000	80.7
rpl20-rps12_p1	rpl20-rps12	1	TTGTTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.464026	68024	68816	862-867	1.000000	77.0
rpl20-rps12_p2	rpl20-rps12	2	TTGTTTCTACGTCTCCGAGC	GCTCTTAGGGGATGTCCTCA	20	20	50.0	55.0	57.7	58.2	0.0	1.1	0.548195	68024	68816	883-888	1.000000	76.3
rpl20-rps12_p3	rpl20-rps12	3	TTGTTTCTACGTCTCCGAGC	TCTTAGGGGATGTCCTCAGC	20	20	50.0	55.0	57.7	58.2	0.0	1.1	0.548195	68024	68816	881-886	1.000000	76.3
rpl20-rps12_p4	rpl20-rps12	4	TGTTTCTACGTCTCCGAGCT	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	58.5	57.9	0.0	0.0	0.589694	68024	68816	861-866	1.000000	76.0
rpl20-rps12_p5	rpl20-rps12	5	TGTTTCTACGTCTCCGAGCT	TCTTAGGGGATGTCCTCAGC	20	20	50.0	55.0	58.5	58.2	0.0	1.1	0.673863	68024	68816	880-885	1.000000	75.3
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCCCTAGTCCTATTCCCA	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	57.1	57.9	0.0	23.0	1.038438	111176	111760	760-779	1.000000	74.8
ndhF-rpl32_p2	ndhF-rpl32	2	CTGGAAGTGGAAGGAAAGGT	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	57.0	57.9	0.0	23.0	1.108381	111176	111760	734-753	1.000000	74.4
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCCCTAGTCCTATTCCCA	GCCCAATACCCCTTCCTTTT	20	20	50.0	50.0	57.1	57.8	0.0	0.0	1.137221	111176	111760	743-762	1.000000	74.1
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGAAGTGGAAGGAAAGGT	GCCCAATACCCCTTCCTTTT	20	20	50.0	50.0	57.0	57.8	0.0	0.0	1.207164	111176	111760	717-736	1.000000	73.6
ndhF-rpl32_p5	ndhF-rpl32	5	AGCCCTAGTCCTATTCCCAT	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	56.9	57.9	0.0	23.0	1.276332	111176	111760	759-778	1.000000	72.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTAAAAGCCTTTT	TTCGAACCGAGATGCTCAAG	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.152624	111920	112358	540-587	1.000000	88.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTAAAAGCCTTTT	TTAGAAGAGAATGTGCCGCC	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.173091	111920	112358	567-614	1.000000	87.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTAAAAGCCTTTT	AAGCACTGCTTCCTAAGAGC	20	20	45.0	50.0	57.9	57.9	7.7	10.7	0.255393	111920	112358	523-570	1.000000	87.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAAGGAAGGGGTATTGGGC	TTCGAACCGAGATGCTCAAG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.251407	111920	112358	557-604	1.000000	87.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAAGGAAGGGGTATTGGGC	TTAGAAGAGAATGTGCCGCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.271875	111920	112358	584-631	1.000000	86.7
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	CTTGAGCATCTCGGTTCGAA	AGAGAGTCCTATCATTTCATCGAC	20	24	50.0	41.7	58.0	57.7	0.0	0.0	4.329628	112440	112510	220-220	1.000000	47.2
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	CTTGAGCATCTCGGTTCGAA	GAGAGTCCTATCATTTCATCGACT	20	24	50.0	41.7	58.0	57.7	0.0	0.0	4.329628	112440	112510	219-219	1.000000	47.2
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	CTTGAGCATCTCGGTTCGAA	AGAGTCCTATCATTTCATCGACT	20	23	50.0	39.1	58.0	56.6	0.0	0.0	4.384590	112440	112510	218-218	1.000000	46.8
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TAGACACGCTGCTCTTAGGA	AGAGAGTCCTATCATTTCATCGAC	20	24	50.0	41.7	57.9	57.7	0.0	0.0	4.450196	112440	112510	247-247	1.000000	46.0
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TAGACACGCTGCTCTTAGGA	GAGAGTCCTATCATTTCATCGACT	20	24	50.0	41.7	57.9	57.7	0.0	0.0	4.450196	112440	112510	246-246	1.000000	46.0
ndhA_p1	ndhA	1	TGCAGCTCGTACTGTTTGTT	GTTTATCGGGCCCAATGCTA	20	20	45.0	50.0	58.1	58.0	8.8	24.9	0.076236	117648	119832	2829-2850	1.000000	81.0
ndhA_p2	ndhA	2	TGCAGCTCGTACTGTTTGTT	TTTATCGGGCCCAATGCTAC	20	20	45.0	50.0	58.1	58.0	8.8	24.9	0.076236	117648	119832	2828-2849	1.000000	81.0
ndhA_p3	ndhA	3	TTGCAGCTCGTACTGTTTGT	GTTTATCGGGCCCAATGCTA	20	20	45.0	50.0	58.1	58.0	8.8	24.9	0.076236	117648	119832	2830-2851	1.000000	81.0
ndhA_p4	ndhA	4	TTGCAGCTCGTACTGTTTGT	TTTATCGGGCCCAATGCTAC	20	20	45.0	50.0	58.1	58.0	8.8	24.9	0.076236	117648	119832	2829-2850	1.000000	81.0
ndhA_p5	ndhA	5	TGCAGCTCGTACTGTTTGTT	GCTACGAGCTTCCGGAATAG	20	20	45.0	55.0	58.1	57.9	8.8	19.2	0.105178	117648	119832	2813-2834	1.000000	80.8
rps15-ycf1_p1	rps15-ycf1	1	TCAACGGATCCCTTGGTTTC	TTCTTTGGCCGACTTGTCAA	20	20	50.0	45.0	57.8	58.0	19.5	0.0	0.239763	121398	121800	540-599	1.000000	83.6
rps15-ycf1_p2	rps15-ycf1	2	TCAACGGATCCCTTGGTTTC	TTTCTTTGGCCGACTTGTCA	20	20	50.0	45.0	57.8	58.0	19.5	0.0	0.239763	121398	121800	541-600	1.000000	83.6
rps15-ycf1_p3	rps15-ycf1	3	CAACGGATCCCTTGGTTTCT	TTCTTTGGCCGACTTGTCAA	20	20	50.0	45.0	57.8	58.0	13.9	0.0	0.245934	121398	121800	539-598	1.000000	83.5
rps15-ycf1_p4	rps15-ycf1	4	CAACGGATCCCTTGGTTTCT	TTTCTTTGGCCGACTTGTCA	20	20	50.0	45.0	57.8	58.0	13.9	0.0	0.245934	121398	121800	540-599	1.000000	83.5
rps15-ycf1_p5	rps15-ycf1	5	TCAACGGATCCCTTGGTTTC	TCTTTGGCCGACTTGTCAAT	20	20	50.0	45.0	57.8	57.7	19.5	0.0	0.464553	121398	121800	539-598	1.000000	81.8
ycf1_p1	ycf1	1	TTTTTGAGGGGTCGTTTATT	CCCCAAAACCAACCTTCCTT	20	20	35.0	50.0	53.0	58.2	0.0	0.0	5.232819	124226	124345	175-175	1.000000	45.9
ycf1_p2	ycf1	2	TTTTTGAGGGGTCGTTTATT	TCACCCCAAAACCAACCTTC	20	20	35.0	50.0	53.0	58.2	0.0	0.0	5.250991	124226	124345	178-178	1.000000	45.8
ycf1_p3	ycf1	3	TTTTTGAGGGGTCGTTTATT	TTCACCCCAAAACCAACCTT	20	20	35.0	45.0	53.0	57.7	0.0	0.0	5.289832	124226	124345	179-179	1.000000	45.8
ycf1_p4	ycf1	4	TTTTTGAGGGGTCGTTTATT	GTTCACCCCAAAACCAACCT	20	20	35.0	50.0	53.0	58.5	0.0	0.0	5.542394	124226	124345	180-180	1.000000	45.8
ycf1_p5	ycf1	5	TTTTTGAGGGGTCGTTTATT	TTTTAACTGTGTGGGGGAGG	20	20	35.0	50.0	53.0	57.3	0.0	0.0	5.702206	124226	124345	219-219	1.000000	44.7