Pi
0.00398
MarkerSeek score
42.50
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | CCGGGATATTGAAGTACAGACC | AGGATCAATGTACAACCTTTCCT | 50.0 | 39.1 | 58.0 | 57.5 | 257 bp | 1.0 | 40.9 |
| ycf1_p2 | AGAAAAAGAGATGTTCCGGGA | AGGATCAATGTACAACCTTTCCT | 42.9 | 39.1 | 56.9 | 57.5 | 272 bp | 1.0 | 40.9 |
| ycf1_p3 | AAGAAAAAGAGATGTTCCGGGA | AGGATCAATGTACAACCTTTCCT | 40.9 | 39.1 | 57.6 | 57.5 | 273 bp | 1.0 | 40.9 |
| ycf1_p4 | CCGGGATATTGAAGTACAGACC | AGGATCAATGTACAACCTTTCC | 50.0 | 40.9 | 58.0 | 56.1 | 257 bp | 1.0 | 40.9 |
| ycf1_p5 | CCGGGATATTGAAGTACAGACC | GGATCAATGTACAACCTTTCCT | 50.0 | 40.9 | 58.0 | 56.1 | 256 bp | 1.0 | 40.9 |