Hotspot

petB

LSC · 78729-80180 · 1452 bp

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Pi 0.0131

MarkerSeek score 55.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1452
variable_sites: 19
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCCCGGCTTTTCAATCCAAA	ACAAATATCTACGGTCTGTCTTCT	45.0	37.5	58.0	57.2	1576 bp	1.0	42.7
petB_p2	GTTCGACCGTGGAATTCCTT	ACAAATATCTACGGTCTGTCTTCT	50.0	37.5	58.2	57.2	1516 bp	1.0	41.2
petB_p3	TCGACCGTGGAATTCCTTTG	ACAAATATCTACGGTCTGTCTTCT	50.0	37.5	58.2	57.2	1514 bp	1.0	41.2
petB_p4	GGCATTCTGGTAGTTCGACC	ACAAATATCTACGGTCTGTCTTCT	55.0	37.5	58.3	57.2	1528 bp	1.0	41.0
petB_p5	GGGCATTCTGGTAGTTCGAC	ACAAATATCTACGGTCTGTCTTCT	55.0	37.5	58.3	57.2	1529 bp	1.0	41.0