Hotspot

ndhK

LSC · 52163-52909 · 747 bp

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Pi 0.00402

MarkerSeek score 49.50

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 747
variable_sites: 3
pi: 0.00402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TGCACAAAAGAAAAGCAGCC	ATCCATGGGCGATGAGTTTC	45.0	50.0	58.1	58.0	885 bp	1.0	79.6
ndhK_p2	TGCACAAAAGAAAAGCAGCC	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.1	58.1	792 bp	1.0	79.2
ndhK_p3	ACAAAAGAAAAGCAGCCTGC	ATCCATGGGCGATGAGTTTC	45.0	50.0	57.8	58.0	882 bp	1.0	78.2
ndhK_p4	GCACAAAAGAAAAGCAGCCT	ATCCATGGGCGATGAGTTTC	45.0	50.0	57.8	58.0	884 bp	1.0	78.2
ndhK_p5	TGCACAAAAGAAAAGCAGCC	GGGCGATGAGTTTCGATGTA	45.0	50.0	58.1	57.8	879 bp	1.0	78.0