Hotspot

atpF

LSC · 12511-13792 · 1282 bp

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Pi 0.00312

MarkerSeek score 60.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1282
variable_sites: 4
pi: 0.00312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTCGTCGGCTCTAATGGTTG	ACGAAGTGATACAAAACGAACTC	50.0	39.1	58.0	57.4	1429-1440 bp	1.0	51.6
atpF_p2	GGCTCTAATGGTTGCCATGA	ACGAAGTGATACAAAACGAACTC	50.0	39.1	57.9	57.4	1423-1434 bp	1.0	51.2
atpF_p3	TTCGTCGGCTCTAATGGTTG	ACGAAGTGATACAAAACGAACT	50.0	36.4	58.0	56.3	1429-1440 bp	1.0	50.9
atpF_p4	GGCTCTAATGGTTGCCATGA	ACGAAGTGATACAAAACGAACT	50.0	36.4	57.9	56.3	1423-1434 bp	1.0	50.5
atpF_p5	TTCGTCGGCTCTAATGGTTG	CGAAGTGATACAAAACGAACTCT	50.0	39.1	58.0	57.2	1428-1439 bp	1.0	49.6