Hotspot

rpl32-trnL-UAG

SSC · 118367-119263 · 897 bp

Back to Hylomecon

Pi 0.0104

MarkerSeek score 57.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 897
variable_sites: 9
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACAAACAAGTAATCAAGTCGTGG	AGCGTGTCTACCGATTTCAC	39.1	50.0	57.6	58.0	925-954 bp	1.0	56.2
rpl32-trnL-UAG_p2	ACAAACAAGTAATCAAGTCGTGG	CAGCGTGTCTACCGATTTCA	39.1	50.0	57.6	58.0	926-955 bp	1.0	56.2
rpl32-trnL-UAG_p3	ACAAACAAGTAATCAAGTCGTGG	TCCTAAGAGCAGCGTGTCTA	39.1	50.0	57.6	57.9	935-964 bp	1.0	55.2
rpl32-trnL-UAG_p4	ACAAACAAGTAATCAAGTCGTGG	TTATAAGATGCCGCCACTCG	39.1	50.0	57.6	57.9	984-1013 bp	1.0	54.8
rpl32-trnL-UAG_p5	ACAAACAAGTAATCAAGTCGTGG	CTAAGAGCAGCGTGTCTACC	39.1	55.0	57.6	57.8	933-962 bp	1.0	54.6