Hotspot

ndhF-rpl32

SSC · 117210-118192 · 983 bp

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Pi 0.0112

MarkerSeek score 53.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 983
variable_sites: 11
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTATGATCCATGCA	TTTATCGCAGCCCAATACCC	42.9	50.0	57.4	58.0	1111-1113 bp	1.0	68.5
ndhF-rpl32_p2	ACGAAAGGTATGATCCATGCA	GCAGCCCAATACCCTTTTCT	42.9	50.0	57.4	58.1	1105-1107 bp	1.0	67.5
ndhF-rpl32_p3	ACGAAAGGTATGATCCATGCA	AGCTTTTATCGCAGCCCAAT	42.9	45.0	57.4	58.2	1115-1117 bp	1.0	66.8
ndhF-rpl32_p4	ACGAAAGGTATGATCCATGCA	AAAGCTTTTATCGCAGCCCA	42.9	45.0	57.4	58.5	1117-1119 bp	1.0	65.0
ndhF-rpl32_p5	ACGAAAGGTATGATCCATGCA	AAGCTTTTATCGCAGCCCAA	42.9	45.0	57.4	58.5	1116-1118 bp	1.0	65.0