Hotspot

psbT-psbN

LSC · 78090-78153 · 64 bp

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Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 2
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACCACCTAAGGTTCCGACTA	GGGTACGCCTTATATACCGC	50.0	55.0	57.4	57.7	173 bp	1.0	73.8
psbT-psbN_p2	ACCACCTAAGGTTCCGACTA	GTTTTACCGGGTACGCCTTA	50.0	50.0	57.4	57.6	181 bp	1.0	73.0
psbT-psbN_p3	ACCACCTAAGGTTCCGACTA	TAAGTTTTACCGGGTACGCC	50.0	50.0	57.4	57.6	184 bp	1.0	73.0
psbT-psbN_p4	ACCACCTAAGGTTCCGACTA	GATCCATTCGAGGAACACGG	50.0	55.0	57.4	58.4	125 bp	1.0	73.0
psbT-psbN_p5	ACCACCTAAGGTTCCGACTA	CGGGTACGCCTTATATACCG	50.0	55.0	57.4	57.5	174 bp	1.0	71.6