Hotspot

psaI-ycf4

LSC · 63042-63483 · 442 bp

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Pi 0.00469

MarkerSeek score 61.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 2
pi: 0.00469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	561-563 bp	1.0	82.0
psaI-ycf4_p2	TAGGCCTAGTATTTCCGGCA	TTGTTTTTCGAGACCCCGTT	50.0	45.0	57.9	58.0	571-573 bp	1.0	81.5
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.9	58.0	555-557 bp	1.0	81.4
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CAGGCCCAGCAGAAATTACT	45.0	50.0	58.0	57.9	580-582 bp	1.0	81.2
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	ACAGGCCCAGCAGAAATTAC	45.0	50.0	58.0	58.2	581-583 bp	1.0	81.0