Hotspot

ndhJ-ndhK

LSC · 52049-52162 · 114 bp

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Pi 0.0439

MarkerSeek score 68.10

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 5
pi: 0.0439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CTAGCCAAGCAGACAAACGA	AGTTCAGTATCTTCCCACGA	50.0	45.0	58.2	55.6	175 bp	1.0	61.4
ndhJ-ndhK_p2	CTAGCCAAGCAGACAAACGA	AAGTTCAGTATCTTCCCACGA	50.0	42.9	58.2	56.4	176 bp	1.0	59.8
ndhJ-ndhK_p3	CTAGCCAAGCAGACAAACGA	AGTTCAGTATCTTCCCACGAA	50.0	42.9	58.2	56.4	175 bp	1.0	59.8
ndhJ-ndhK_p4	CTTGACTAGCCAAGCAGACA	AGTTCAGTATCTTCCCACGA	50.0	45.0	57.5	55.6	180 bp	1.0	59.3
ndhJ-ndhK_p5	ACTAGCCAAGCAGACAAACG	AGTTCAGTATCTTCCCACGA	50.0	45.0	58.5	55.6	176 bp	1.0	59.1