Hotspot

trnT-UGU-trnL-UAA

LSC · 49140-49834 · 695 bp

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Pi 0.0159

MarkerSeek score 68.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 11
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	768-770 bp	1.0	78.8
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	756-758 bp	1.0	78.6
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	765-767 bp	1.0	78.4
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	50.0	50.0	57.8	57.8	800-802 bp	1.0	77.9
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	788-790 bp	1.0	77.7