Hotspot

ycf3-trnS-GGA

LSC · 46990-47727 · 738 bp

Back to Hylomecon

Pi 0.00408

MarkerSeek score 61.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 3
pi: 0.00408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTCTCCGGAAGTTGTCGG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.1	58.1	900-903 bp	1.0	78.8
ycf3-trnS-GGA_p2	TTTTCTCCGGAAGTTGTCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	921-924 bp	1.0	78.3
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCGGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.8	58.1	907-910 bp	1.0	78.3
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCGGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.8	58.1	906-909 bp	1.0	78.3
ycf3-trnS-GGA_p5	TTTTCTCCGGAAGTTGTCGG	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.1	57.8	871-874 bp	1.0	78.0