Hotspot

petN-psbM

LSC · 30103-31295 · 1193 bp

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Pi 0.0101

MarkerSeek score 62.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1193
variable_sites: 12
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	GCCACTGCACTGTTCATTCT	45.0	50.0	58.0	58.8	1341-1347 bp	1.0	75.1
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	GCCACTGCACTGTTCATTCT	50.0	50.0	57.8	58.8	1347-1353 bp	1.0	73.7
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TGCCACTGCACTGTTCATTC	45.0	50.0	58.0	59.0	1342-1348 bp	1.0	72.8
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCCACTGCACTGTTCATTCT	50.0	50.0	57.6	58.8	1287-1293 bp	1.0	72.4
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TGCCACTGCACTGTTCATT	45.0	47.4	58.0	57.9	1342-1348 bp	1.0	72.3