Hotspot

ycf1

SSC · 122823-128228 · 5406 bp

Back to Hygrophila

Pi 0.0246

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5406
variable_sites: 132
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTCAAAATTATGAGTGAAGGT	TGGATGTCGAAAGAGGAAACA	26.1	42.9	52.8	57.2	181-187 bp	1.0	47.0
ycf1_p2	TTTTTCAAAATTATGAGTGAAGGT	TGGATGTCGAAAGAGGAAACA	25.0	42.9	53.6	57.2	182-188 bp	1.0	46.9
ycf1_p3	TTTTCAAAATTATGAGTGAAGGT	TTGGATGTCGAAAGAGGAAACA	26.1	40.9	52.8	57.9	182-188 bp	1.0	46.9
ycf1_p4	TTTTTCAAAATTATGAGTGAAGGT	TTGGATGTCGAAAGAGGAAACA	25.0	40.9	53.6	57.9	183-189 bp	1.0	46.9
ycf1_p5	TTTTCAAAATTATGAGTGAAGGT	CGAAATTGGATGTCGAAAGAGG	26.1	45.5	52.8	57.7	187-193 bp	1.0	46.7