Hotspot

trnL-UAG-ccsA

SSC · 113263-113356 · 94 bp

Back to Hygrophila

Pi 0.0449

MarkerSeek score 77.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 4
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	57.9	53.2	174-178 bp	1.0	44.1
trnL-UAG-ccsA_p2	GTGAAATCGGTAGACACGCT	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	58.0	53.2	184-188 bp	1.0	43.9
trnL-UAG-ccsA_p3	TGAAATCGGTAGACACGCTG	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	58.0	53.2	183-187 bp	1.0	43.9
trnL-UAG-ccsA_p4	ACAAGCCGCTATGGTGAAAT	TGCTCTAAGGTTGAAAATATCA	45.0	31.8	57.9	53.2	197-201 bp	1.0	43.6
trnL-UAG-ccsA_p5	GAGTTCAAGACAAGCCGCTA	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	57.9	53.2	206-210 bp	1.0	43.5