Hotspot

accD-psaI

LSC · 59460-60206 · 747 bp

Back to Hygrophila

Pi 0.0427

MarkerSeek score 86.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 30
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTGCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	59.0	58.0	859-869 bp	1.0	78.2
accD-psaI_p2	GCACGCCTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	59.0	58.0	857-867 bp	1.0	78.2
accD-psaI_p3	CTGCACGCCTTCTTTCCTTT	TTGCCGGAAATACTAAGCCC	50.0	50.0	59.0	58.0	850-860 bp	1.0	78.1
accD-psaI_p4	GCACGCCTTCTTTCCTTTGA	TTGCCGGAAATACTAAGCCC	50.0	50.0	59.0	58.0	848-858 bp	1.0	78.1
accD-psaI_p5	TGCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	47.4	45.0	57.9	58.0	858-868 bp	1.0	77.7