Hotspot

ndhC-trnV-UAC

LSC · 50861-52028 · 1168 bp

Back to Hygrophila

Pi 0.0416
MarkerSeek score 75.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1168
variable_sites
46
pi
0.0416
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 27.3 50.0 52.7 57.8 1171-1224 bp 1.0 47.1
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 27.3 55.0 52.7 57.5 1170-1223 bp 1.0 47.1
ndhC-trnV-UAC_p3 ATCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 1172-1225 bp 1.0 47.1
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAAAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 1171-1224 bp 1.0 47.1
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 27.3 55.0 52.7 58.7 1159-1212 bp 1.0 47.1