Hotspot

trnG-GCC-trnfM-CAU

LSC · 36841-37009 · 169 bp

Back to Hygrophila

Pi 0.075
MarkerSeek score 84.50
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
169
variable_sites
12
pi
0.075
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 226-235 bp 1.0 86.5
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 227-236 bp 1.0 86.5
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 262-271 bp 1.0 85.6
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 263-272 bp 1.0 85.6
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC TGACTTCTTCTCTTCAGCGC 50.0 50.0 58.1 57.9 283-292 bp 1.0 84.8