Hotspot

psbI-trnS-GCU

LSC · 7971-8082 · 112 bp

Back to Hygrophila

Pi 0.306

MarkerSeek score 77.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 33
pi: 0.306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TAGGGTCTCGCACAAAATCG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	489-495 bp	1.0	88.2
psbI-trnS-GCU_p2	TAGGGTCTCGCACAAAATCG	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	490-496 bp	1.0	88.2
psbI-trnS-GCU_p3	ACCCGTTTCCAGTGAAAGAC	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	520 bp	0.5	59.7
psbI-trnS-GCU_p4	TGACCCGTTTCCAGTGAAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	522 bp	0.5	59.7
psbI-trnS-GCU_p5	ACCCGTTTCCAGTGAAAGAC	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	521 bp	0.5	59.7