Hotspot

trnK-UUU-rps16

LSC · 4228-4676 · 449 bp

Back to Hygrophila

Pi 0.055

MarkerSeek score 75.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 23
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.5	58.2	542-559 bp	1.0	82.1
trnK-UUU-rps16_p2	TACCGTTGAGTTAGCAACCC	GTGCTCAACCTACAGGAACT	50.0	50.0	57.5	57.2	541-558 bp	1.0	76.9
trnK-UUU-rps16_p3	TCTACCGTTGAGTTAGCAACC	GGTGCTCAACCTACAGGAAC	47.6	55.0	57.7	58.2	544-561 bp	1.0	75.3
trnK-UUU-rps16_p4	ACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	52.6	55.0	57.7	58.2	541-558 bp	1.0	75.3
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	TCCTTGAGAAAGGTGCTCAAC	50.0	47.6	57.5	58.1	553-570 bp	1.0	74.4