Hotspot

trnH-GUG-psbA

LSC · 63-531 · 469 bp

Back to Hygrophila

Pi 0.0792
MarkerSeek score 81.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
469
variable_sites
32
pi
0.0792
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CGTGCTAACCTTGGTATGGA 55.0 50.0 58.1 57.3 568-584 bp 1.0 81.9
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCGTGCTAACCTTGGTATGG 55.0 50.0 58.1 57.3 569-585 bp 1.0 81.9
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 582-598 bp 1.0 81.8
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCGTGCTAACCTTGGTATGG 50.0 50.0 57.9 57.3 583-599 bp 1.0 81.8
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 581-597 bp 1.0 81.8