Hotspot

ycf1

SSC · 125532-131084 · 5553 bp

Back to Hydrostachys

Pi 0.0317

MarkerSeek score 59.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5553
variable_sites: 261
pi: 0.0317
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TACTACACGCCGAGCTTTTC	ACAGCCGATTTGAAAAAGCC	50.0	45.0	58.0	57.6	863-866 bp	0.666667	66.1
ycf1_p2	TACTACACGCCGAGCTTTTC	CGGGGAACAAGCAAAAACAA	50.0	45.0	58.0	57.7	492 bp	0.333333	50.8
ycf1_p3	CGCGCATTGAACCTTTGATT	CGGGGAACAAGCAAAAACAA	45.0	45.0	58.0	57.7	631 bp	0.333333	50.8
ycf1_p4	ATGCGCATTTGCTTGAAACA	CGGGGAACAAGCAAAAACAA	40.0	45.0	57.8	57.7	681 bp	0.333333	49.7
ycf1_p5	TGCGCATTTGCTTGAAACAT	CGGGGAACAAGCAAAAACAA	40.0	45.0	57.8	57.7	680 bp	0.333333	49.7