Hotspot

rpl32-trnL-UAG

SSC · 114965-115694 · 730 bp

Back to Hydrostachys

Pi 0.0736

MarkerSeek score 81.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 730
variable_sites: 77
pi: 0.0736
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCACTAAAGGGCTTTGCAT	TATCAAATCATGTGCCGCCA	45.0	45.0	58.1	58.0	907-948 bp	0.666667	70.4
rpl32-trnL-UAG_p2	AGCACTAAAGGGCTTTGCAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	854-895 bp	0.666667	70.0
rpl32-trnL-UAG_p3	AAAGCATATTGGGCAGCACT	TATCAAATCATGTGCCGCCA	45.0	45.0	58.1	58.0	921-962 bp	0.666667	69.7
rpl32-trnL-UAG_p4	AGAAAGCATATTGGGCAGCA	TATCAAATCATGTGCCGCCA	45.0	45.0	57.9	58.0	923-964 bp	0.666667	69.7
rpl32-trnL-UAG_p5	AGCACTAAAGGGCTTTGCAT	ACGAGAAGGGCTACAGCTAA	45.0	50.0	58.1	58.2	1549-1591 bp	0.666667	68.8