Hotspot

ndhF-rpl32

SSC · 114153-114799 · 647 bp

Back to Hydrostachys

Pi 0.0726
MarkerSeek score 85.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
647
variable_sites
66
pi
0.0726
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCTTTTTGACACGCACTTG ATGCAAAGCCCTTTAGTGCT 45.0 45.0 58.1 58.1 1262-1285 bp 1.0 86.1
ndhF-rpl32_p2 TTTTTGACACGCACTTGCTG ATGCAAAGCCCTTTAGTGCT 45.0 45.0 58.1 58.1 1259-1282 bp 1.0 86.1
ndhF-rpl32_p3 GCAAGAGGTCGTGTAAACCA ATGCAAAGCCCTTTAGTGCT 50.0 45.0 58.1 58.1 1240-1263 bp 1.0 85.9
ndhF-rpl32_p4 TGCAAGAGGTCGTGTAAACC ATGCAAAGCCCTTTAGTGCT 50.0 45.0 58.1 58.1 1241-1264 bp 1.0 85.9
ndhF-rpl32_p5 GCCCATACACGACGAAGATT ATGCAAAGCCCTTTAGTGCT 50.0 45.0 58.1 58.1 869-873 bp 0.666667 70.4