Hotspot

trnP-GGG-psaJ

LSC · 67813-68348 · 536 bp

Back to Hydrostachys

Pi 0.0361
MarkerSeek score 73.60
Primer available yes
Label trnP-GGG-psaJ

Feature Metadata

label_name
trnP-GGG-psaJ
feature_type
igs
region
LSC
length_bp
536
variable_sites
28
pi
0.0361
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-psaJ_p1 ACCCGTGACATTTTGTACCC CTGCTAAAGCTCCGAACCAT 50.0 50.0 58.1 58.0 660-673 bp 1.0 83.9
trnP-GGG-psaJ_p2 CCCGTGACATTTTGTACCCA CTGCTAAAGCTCCGAACCAT 50.0 50.0 58.1 58.0 659-672 bp 1.0 83.9
trnP-GGG-psaJ_p3 ACAGGATTTGAACCCGTGAC CTGCTAAAGCTCCGAACCAT 50.0 50.0 58.1 58.0 671-684 bp 1.0 83.7
trnP-GGG-psaJ_p4 CAGGATTTGAACCCGTGACA CTGCTAAAGCTCCGAACCAT 50.0 50.0 58.1 58.0 670-683 bp 1.0 83.7
trnP-GGG-psaJ_p5 TGACAGGATTTGAACCCGTG CTGCTAAAGCTCCGAACCAT 50.0 50.0 58.1 58.0 673-686 bp 1.0 83.7