Hotspot

atpB-rbcL

LSC · 55444-56343 · 900 bp

Back to Hydrostachys

Pi 0.0399

MarkerSeek score 71.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 52
pi: 0.0399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACGTTCACCTACTCCTCCAA	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1739-1768 bp	1.0	84.0
atpB-rbcL_p2	GTACAGTTTTGCCCACTCCA	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1665-1694 bp	1.0	84.0
atpB-rbcL_p3	ACGTTCACCTACTCCTCCAA	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1727-1756 bp	1.0	83.8
atpB-rbcL_p4	ACGTTCACCTACTCCTCCAA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2170-2199 bp	1.0	83.3
atpB-rbcL_p5	GTACAGTTTTGCCCACTCCA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2096-2125 bp	1.0	83.2