Hotspot

trnS-GGA-rps4

LSC · 46321-46664 · 344 bp

Back to Hydrostachys

Pi 0.0514

MarkerSeek score 81.70

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 25
pi: 0.0514
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TGGGTTGGTTTGAAAATCAATGA	50.0	34.8	58.1	57.4	427-444 bp	1.0	58.9
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	TGGGTTGGTTTGAAAATCAATGA	50.0	34.8	58.4	57.4	426-443 bp	1.0	56.6
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	TGGGTTGGTTTGAAAATCAATGAA	50.0	33.3	58.1	58.0	427-444 bp	1.0	55.8
trnS-GGA-rps4_p4	AGGCTTTTGTTTACCGAGGG	TTGGGTTGGTTTGAAAATCAATGA	50.0	33.3	58.1	58.0	428-445 bp	1.0	55.8
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	TGGGTTGGTTTGAAAATCAATG	50.0	36.4	58.1	56.0	427-444 bp	1.0	55.5