Hotspot

rpoB-trnC-GCA

LSC · 26564-27971 · 1408 bp

Back to Hydrostachys

Pi 0.0436

MarkerSeek score 76.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1408
variable_sites: 86
pi: 0.0436
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAATACTGGATCCCCACCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	2132-2187 bp	1.0	86.7
rpoB-trnC-GCA_p2	ATTCGAACCCATAGCTGCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1985-2040 bp	1.0	86.7
rpoB-trnC-GCA_p3	ATTCGAACCCATAGCTGCTG	AACTTTGCAGAGCAGAACGA	50.0	45.0	58.0	58.0	2552-2610 bp	1.0	86.5
rpoB-trnC-GCA_p4	AAATACTGGATCCCCACCCA	AACTTTGCAGAGCAGAACGA	50.0	45.0	58.0	58.0	2699-2757 bp	1.0	86.4
rpoB-trnC-GCA_p5	AAATACTGGATCCCCACCCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	2556-2614 bp	1.0	85.7