Hotspot

atpH-atpI

LSC · 13120-14263 · 1144 bp

Back to Hydrostachys

Pi 0.0452

MarkerSeek score 81.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 72
pi: 0.0452
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GGGGAATCTATGGAGGGTCA	45.0	55.0	58.0	57.9	1072-1203 bp	1.0	87.8
atpH-atpI_p2	TGGTCCAATAGAAGCAAGCC	GGGGAATCTATGGAGGGTCA	50.0	55.0	57.9	57.9	1112-1243 bp	1.0	86.9
atpH-atpI_p3	TGCCAATCCAGCAGCAATAA	GGGGAATCTATGGAGGGTCA	45.0	55.0	58.2	57.9	1088-1219 bp	1.0	86.8
atpH-atpI_p4	AACGGAAGCGGCAGAAATAA	GGGGAATCTATGGAGGGTCA	45.0	55.0	58.2	57.9	1070-1201 bp	1.0	86.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GGGGAATCTATGGAGGGTCA	45.0	55.0	58.2	57.9	1073-1204 bp	1.0	86.6