Hotspot

trnR-UCU-atpA

LSC · 9479-9638 · 160 bp

Back to Hydrostachys

Pi 0.0708

MarkerSeek score 79.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 17
pi: 0.0708
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	ATGAAACAAGTAGCGGGCAA	50.0	45.0	58.0	58.1	808-839 bp	1.0	84.8
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	ATGAAACAAGTAGCGGGCAA	55.0	45.0	58.1	58.1	811-842 bp	1.0	84.6
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	TTTCTGTTTCGAGAGTGGGC	50.0	50.0	58.0	58.1	849-880 bp	1.0	84.4
trnR-UCU-atpA_p4	CCTAGCCTTCCAAGCTAACG	TTTCTGTTTCGAGAGTGGGC	55.0	50.0	58.1	58.1	852-883 bp	1.0	84.2
trnR-UCU-atpA_p5	AGCCTTCCAAGCTAACGATG	CGAAGCTTATCCAGGGGATG	50.0	55.0	58.0	57.8	1082-1113 bp	1.0	83.1